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JKMRS Volume 24, No 3, pp 70, Backbone NMR assignments of the ...
2020년 09월 20일 / 조회수: 489

Backbone NMR assignments of the anti-CRISPR AcrIIA5 from phages infecting Streptococcus thermophilus

 

 

So Young An1, Eun-Hee Kim2, Euiyoung Bae1, and Jeong-Yong Suh1,*

 

 

1Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea,

2Center for Research Equipment, Korea Basic Science Institute, Ochang 28119, Republic of Korea

 

 

 

 

Received Sep 10, 2020; Revised Sep 17, 2020; Accepted Sep 17, 2020

 


Abstract The CRISPR-Cas system provides an adaptive immunity for bacteria and archaea against invading phages or foreign plasmids. In the type II CRISPR-Cas system, a single effector protein Cas9 and a guide RNA form an RNA-guided endonuclease complex that can degrade DNA targets of foreign origin. To avoid the Cas9-mediated destruction, phages evolved anti-CRISPR (Acr) proteins that neutralize the host bacterial immunity by inactivating the CRISPR-Cas system. Here we report the backbone 1H, 15N, and 13C resonance assignments of AcrIIA5 that inhibits the endonuclease activity of type II-A Streptococcus thermophilus Cas9 and also Streptococcus pyogenesis Cas9 using triple resonance nuclear magnetic resonance spectroscopy. The backbone chemical shifts of AcrIIA5 predict a disordered region at the N-terminus, followed by an abbbbabbb fold.

 

Keywords AcrIIA5, anti-CRISPR, Cas9, NMR spectroscopy

 

* Address correspondence to: Jeong-Yong Suh, Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea, Tel: 82-2-880-4879; E-mail: jysuh@snu.ac.kr


 

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