JKMRS Volume 24, No 3, pp 70, Backbone NMR assignments of the ... | |
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2020년 09월 20일 / 조회수: 489 | |
Backbone NMR assignments of the anti-CRISPR
AcrIIA5 from phages infecting
Streptococcus thermophilus
So Young An1, Eun-Hee Kim2, Euiyoung
Bae1, and Jeong-Yong Suh1,*
1Department of Agricultural Biotechnology and
Research Institute of Agriculture and Life Sciences, Seoul National University,
Seoul 08826, Republic of Korea, 2Center for Research Equipment, Korea Basic Science
Institute, Ochang 28119, Republic of Korea
Received Sep 10, 2020; Revised
Sep 17, 2020; Accepted Sep 17, 2020
Abstract The
CRISPR-Cas system provides an adaptive immunity for bacteria and archaea against
invading phages or foreign plasmids. In the type II CRISPR-Cas system, a single
effector protein Cas9 and a guide RNA form an RNA-guided endonuclease complex
that can degrade DNA targets of foreign origin. To avoid the Cas9-mediated
destruction, phages evolved anti-CRISPR (Acr) proteins that neutralize the host
bacterial immunity by inactivating the CRISPR-Cas system. Here we report the backbone
1H, 15N, and 13C resonance assignments of
AcrIIA5 that inhibits the endonuclease activity of type II-A Streptococcus thermophilus Cas9 and also
Streptococcus pyogenesis Cas9 using triple
resonance nuclear magnetic resonance spectroscopy. The backbone chemical shifts
of AcrIIA5 predict a disordered region at the N-terminus, followed by an abbbbabbb fold.
Keywords AcrIIA5, anti-CRISPR, Cas9, NMR spectroscopy
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첨부파일 | 02-JKMRS-JY_Suh.pdf |