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JKMRS Volume 24, No 2, pp 43, Monitoring fibrillation of the p...
2020년 06월 20일 / 조회수: 434

Monitoring fibrillation of the pathogenic huntingtin protein using NMR

 

 

Min-Duk Seo1,2,*

 

 

1Department of Molecular Science and Technology, Ajou University, Suwon, Gyeonggi 16499, Republic of Korea

2College of Pharmacy, Ajou University, Suwon, Gyeonggi 16499, Republic of Korea

 

 

Received June 12, 2020; Revised June 15, 2020; Accepted June 16, 2020

 


Abstract Huntington’s disease (HD) is an inherited neurodegenerative disease caused by abnormal polyglutamine (polyQ) expansion in the huntingtin protein (Htt). There is no cure for HD so far. Although exact molecular mechanism of HD pathogenesis is still elusive, fibril formation of the expanded Htt is linked to the toxicity. In this study, we prepared the expanded Htt containing 46 glutamines, and induced the fibrillation by proteolytic cleavage. Fibrillation of the pathogenic Htt has been monitored by time course NMR experiment. The NMR-based monitoring method could be widely used to screen the candidates to inhibit the fibrillation of the pathogenic Htt.

 

Keywords Huntington’s disease, huntingtin protein, polyQ, fibrillation, NMR 

 


* Address correspondence to: Min-Duk Seo, Department of Molecular Science and Technology & College of Pharmacy, Ajou University, Suwon, Gyeonggi 16499, Republic of Korea, Tel: 82-31-219-3450; Fax: 82-31-219-3435; E-mail: mdseo@ajou.ac.kr

 

 

첨부파일 2_Seo_Htt_for.pdf