투고논문
| JKMRS Volume 24, No 1, pp 1, Backbone NMR assignments of the F... | |
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| 2020년 03월 20일 / 조회수: 1004 | |
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Backbone NMR assignments of the FAS1-3/FAS1-4 domains of transforming growth factor-beta-induced protein
Dong-Hoon Kang1, ξ, Jong-Jae Yi2, ξ, Dae-Won Sim3, Jung-Wook Park1, Sung-Hee Lee1, Eun-Hee Kim4, Young-Ho Jeon5, Woo Sung Son2, Hyung-Sik Won3,*, and Ji-Hun Kim1,*
1College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28160, Republic of Korea 2Department of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Pochen, Gyeonggi 11160, Republic of Korea 3Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk 27478, Republic of Korea 4Research Center for Bioconvergence Analysis, Korea Basic Science Institute, 162 Yeongudanji-Ro, Ochang-Eup, Cheongju-Si, Chungcheongbuk-Do 28119, Republic of Korea 5Korea University College of Pharmacy Sejong-ro, Jochiwon, Yeonggi-gun, Chungnam 339-700, Republic of Korea
Received March 9, 2020; Revised March 15, 2020; Accepted March 16, 2020
Abstract An extracellular matrix protein, transforming growth factor-beta-induced protein (TGFBIp/βig-h3), which is induced by transforming growth factor-β in the human cornea, skin, and matrix of many connective tissues, is associated with the adhesion, migration, proliferation, and differentiation of various cells. TGFBIp contains four homologous repeat domains, known as FAS1 domains, where certain mutations have been considered to cause corneal dystrophies. In this study, backbone NMR assignments of FAS1-3/FAS1-4 tandem domain were obtained and compared with those previously known for the isolated FAS1-4 domain. The results corroborate in solution the inter-domain interaction between FAS1-3 and FAS1-4 in TGFBIp.
Keywords Blvrb, dialysis using micro-dialyzer, 1-dimensional HSQC experiment, 15N-labeled protein, protein refolding
*Address correspondence to: Ji-Hun Kim and Hyung-Sik Won, College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28160, Republic of Korea, Tel.: +82 43 249 1343; Email: nmrjhkim@cbnu.ac.kr / 3Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk 27478, Republic of Korea, Tel: +82 43 840 3589; E-mail: wonhs@konkuk.ac.kr, ξ Equally contributed to this work
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| 첨부파일 | 1_JKMRS_Kang and Yi et al.pdf |