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JKMRS Volume 23, No 3, pp 61, NMR hydrogen exchange study of m...
2019년 09월 22일 / 조회수: 552

NMR hydrogen exchange study of miR156:miR156* duplexes

 

 

Na-Hyun Kim, Seo-Ree Choi, Ho-Seong Jin, Yeo-Jin Seo, and Joon-Hwa Lee*

 

 

Department of Chemistry and RINS, Gyeongsang National University, Jinju, Gyeongnam 52828, Republic Korea

 

 

 

 

Received Aug 13, 2019; Revised Sep 10, 2019; Accepted Sep 15, 2019

 


Abstract RNAs exhibit distinct structural and dynamic features required for proper function. The hydrogen-bonded imino protons of RNAs are a probe of the conformational transition and dynamic feature. MicroRNAs originate from primary transcripts containing hairpin structures. The levels of mature miR156 influence the flowering time of plants. To understand the molecular mechanism of biological function of miR156:miR156* duplex, we performed hydrogen exchange study on the model RNAs mimicking two phenotypes of miR156:miR156*, miR156a:miR156a* (m-miR156a) and miR156g:miR156g* (m-miR156g) duplexes. This study found that the internal bulge of m-miR156a destabilized the neighboring base-pairs, whereas the bulge structure of m-miR156g did not affect the thermal stabilities of the neighboring base-pairs.

 

Keywords microRNA, miRNA156, flowering time, hydrogen exchange, NMR 

 


* Address correspondence to: Joon-Hwa Lee, Department of Chemistry and RINS, Gyeongsang National University, Jinju, Gyeongnam 52828, Republic of Korea, Tel: 82-55-772-1490; E-mail: joonhwa@gnu.ac.kr



 

 

첨부파일 1_KimJH_final.pdf