투고논문

In-cell nuclear magnetic resonance spectroscopy for studying intermolecular interactions

2 Protein Structure Group, Division of Bioconvergence Analysis, Korea Basic Science Institute, Chungcheongbuk-do, 28119, Republic of Korea

3 Bio-Analytical Science, University of Science and Technology, Daejeon 34113, Republic of Korea

Studies on the interactions of proteins with partner molecules at the atomic resolution are essential for understanding the biological function of proteins in cells and for developing drug molecules. Solution NMR spectroscopy has shown remarkably useful capability for investigating properties on the weak to strong intermolecular interactions in both diluted and crowded solution such as cell lysates. Of note, the state-of-the-art in-cell NMR method has made it possible to obtain atomistic information on natures of intermolecular interactions between target proteins with partner molecules in living cells. In this mini-review, we comprehensively describe the several technological advances and developments in the in-cell NMR spectroscopy.