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JKMRS Volume 23, No 1, pp 26, Recent advances in NMR-based str...
2019년 03월 20일 / 조회수: 563

Recent advances in NMR-based structural characterization of αB-crystallin and its potential role in human diseases


Srinivasan Muniyappan and Jin Hae Kim*


Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Daegu 42988, Republic of Korea


Received Mar 18, 2019; Revised Mar 19, 2019; Accepted Mar 20, 2019


Abstract

αB-crystallin (αBC) is a member of a small heat-shock protein (sHSP) superfamily and plays a predominant role in cellular protein homeostasis network by rescuing misfolded proteins from irreversible aggregation. αBC assembles into dynamic and polydisperse high molecular weight complexes containing 12 to 48 monomers; this variable stereochemistry of αBC has been linked to quaternary subunit exchange and its chaperone activity. The chaperone activity of αBC poses great potential as therapeutic agents for various neurodegenerative diseases. In this mini-review, we briefly outline the recent advancement in structural characterization of αBCs and its potential role to inhibit protein misfolding and aggregation in various human diseases. In particular, nuclear magnetic resonance (NMR) spectroscopy and its complimentary techniques have contributed much to elucidate highly-dynamic nature of αBCs, among which notable advancements are discussed in detail. We highlight the importance of resolving the structural details of various αBC oligomers, their quaternary dynamics, and structural heterogeneity.


*Address correspondence to: Jin Hae Kim, Department of New Biology, Daegu Gyeongbuk Institute of Science &Technology, Daegu 42988, Republic of Korea, Tel: 82-53-785-1770, E-mail: jinhaekim@dgist.ac.kr

첨부파일 5_Muniyappan et al.pdf