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JKMRS Volume 22, No 4, pp 149, 1H NMR metabolomics study for d...
2018년 12월 20일 / 조회수: 585

1H NMR metabolomics study for diabetic neuropathy and diabetes


Ja-Shil Hyun1,ǂ, Jiwon Yang2,ǂ, Hyun-Hwi Kim1, Yeong-Bae Lee2,*, and Sung Jean Park1,*


1 College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea
2 Department of Neurology, Gil Medical Center, Gachon University College of Medicine, 21 Namdong-daero 774beon-gil, Namdong-gu, Incheon 21565, Republic of Korea


Received Dec 02, 2018; Revised Dec 17, 2018; Accepted Dec 18, 2018


Abstract

Diabetes is known to be one of common causes for several types of peripheral nerve damage. Diabetic neuropathy (DN) is a significant complication lowering the quality of life that can be frequently found in diabetes patients. In this study, the metabolomic characteristic of DN and Diabetes was investigated with NMR spectroscopy. The sera samples were collected from DN patients, Diabetes patients, and healthy volunteers. Based on the pair-wise comparison, three metabolites were found to be noticeable: glucose, obviously, was upregulated both in DN patients (DNP) and Diabetes. Citrate is also increased in both diseases. However, the dietary nutrient and biosynthesized metabolite from glucose, ascorbate, was elevated only in DNP, compared to healthy control. The multivariate model of OPLS-DA clearly showed the group separation between healthy control-DNP and healthy control-Diabetes. The most significant metabolites that contributed the group separation included glucose, citrate, ascorbate, and lactate. Lactate did not show the statistical significance of change in t-test while it tends to down-regulated both in DNP and Diabetes. We also conducted the ROC curve analysis to make a multivariate model for discrimination of healthy control and diseases with the identified three metabolites. As a result, the discrimination model between healthy control and DNP (or Diabetes) was successful while the model between DNP and Diabetes was not satisfactory for discrimination. In addition, multiple combinations of lactate and citrate in the OPLS-DA model of healthy control and diabetes group (DNP + Diabetes patients) gave good ROC value of 0.952, which imply these two metabolites could be used for diagnosis of Diabetes without glucose information.


ǂ These authors contributed equally.
* Address correspondence to: Sung Jean Park or Yeong-Bae Lee, College of Pharmacy, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon, Republic of Korea, Tel: 82-32-820-4957; E-mail: psjnmr@gachon.ac.kr for Sung Jean Park or Department of Neurology, Gil Medical Center, Gachon University College of Medicine, Republic of Korea; E-mail: yeongbaelee@gachon.ac.kr for Yeong-Bae Lee.

첨부파일 12-JKMRS_ParkSJ.pdf