JKMRS Volume 22, No 4, pp 144, Characterization of pH-dependen... | |
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2018년 12월 20일 / 조회수: 588 | |
Characterization of pH-dependent structural properties of hydrolase PncA using NMR
Received Dec 06, 2018; Revised Dec 15, 2018; Accepted Dec 17, 2018 Abstract Catalytic enzyme Pyrazinamidase (PncA) from Mycobacterium tuberculosis can hydrolyze substrate pyrazinamide (PZA) to pyrazoic acid (POA) as active form of compound. Using NMR spectroscopy, pH-dependent catalytic properties were monitored including metal binding mode during converting PZA to POA. There seems to be a conformational change through zinc binding in active site from the perturbation of peak intensities in series of 2D HSQC spectra the conformation changes through zinc binding. ǂ These authors contributed equally. |
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첨부파일 | 11-JKMRS_SonWS_2.pdf |