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JKMRS Volume 22, No 2, pp 26; Identification of Xanthium Sibir...
2018년 06월 21일 / 조회수: 636

Identification of Xanthium Sibiricum Components using LC-SPE-NMR-MS Hyphenated System

 

 

Ji Soo Sohn1, Youngae Jung1,2, Ji Soo Han1, Geum-Sook Hwang1,3,*


 

1Integtrated Metabolomics Research Group, Western Seoul Center, Korea Basic Science Institute, Seoul 03759, Korea

2Department of Life Science, Ewha Womans University, Seoul 03760, Korea

3Department of Chemistry & Nanoscience, Ewha Womans University, Seoul, Republic of Korea


Abstract Xanthium sibiricum is used as a traditional folk medicine for the treatment of cancer, fever, headache, nasal sinusitis, and skin pruritus. This study aimed to identify components from Xanthium sibiricumextracts using an SPE-800MHz NMR-MS hyphenated system. The simultaneous acquisition of MS and NMR spectra from the same chromatographic peaks significantly increases the depth of information acquired for the compound and allows the elucidation of structures that would not be possible using MS or NMR data alone. LC -NMR analysis was conducted using a HPLC separation system coupled to 800 MHz spectrometerequipped with a cryoprobe, and a SPE unit was used to automatically trap chromatographic peaks using a HPLC pump. LC-MS analysis was conducted with a Q-TOF MS instrument using ESI ionization in the negative ion mode. Using the hyphenated analysis, several secondary metabolites were identified, such as 3',5'-O-dicaffeoylquinic acid, 1',5'-O-dicaffeoyl- quinic acid, and ethyl caffeate. These results demonstrate that the SPE-800MHz NMR-MS hyphenated system can be used to identify metabolites within natural products that have complex mixtures. 

 

 

첨부파일 1.JKMRS_Hwang.pdf