투고논문

NMR Study on the Preferential Binding of the Zα Domain of Human ADAR1 to CG-repeat DNA Duplex

Ae-Ree Lee, Seo-Ree Choi, Yeo-Jin Seo, and Joon-Hwa Lee*

Department of Chemistry and RINS, Gyeongsang National University, Jinju, Gyeongnam 52828, South Korea

Abstract The Z-DNA domain of human ADAR1 (Zα_ADAR1) produces B-Z junction DNA through preferential binding to the CG-repeat segment and destabilizing the neighboring AT-rich region.⁹ However, this study could not answer the question of how many base-pairs in AT-rich region are destabilized by binding of Zα_ADAR1. Thus, we have performed NMR experiments of Zα_ADAR1 to the longer DNA duplex containing an 8-base-paired (8-bp) CG-repeat segment and a 12-bp AT-rich region. This study revealed that Zα_ADAR1 preferentially binds to the CG-repeat segment rather than AT-rich region in a long DNA and then destabilizes at least 6 base-pairs in the neighboring AT-rich region for efficient B-Z transition of the CG-repeat segment.