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JKMRS Volume 21, No 1, pp 20; Backbone Assignment of Phosphor...
2017년 03월 20일 / 조회수: 260

Backbone Assignment of Phosphorylated Cytoplasmic Domain B of Mannitol Transporter IIMtl in Thermoanaerobacter Tengcongensis

 

 

Ko On Lee and Jeong-Yong Suh*

 

 

Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea

 

 Abstract The cytoplasmic domains A and B of the mannitol transporter enzyme IIMtl are covalently linked in Escherichia coli, but separately expressed in Thermoanaerobacter Tengcongensis. The phosphorylation of domain B (TtIIBMtl) substantially increases the binding affinity to the domain A (TtIIAMtl) in T. Tengcongensis. To understand the structural basis of the enhanced domain-domain interaction by protein phosphorylation, we obtained NMR backbone assignments of the phospho-TtIIBMtl using a standard suite of triple resonance experiments. Our results will be useful to monitor chemical shift changes at the active site of phosphorylation and the binding interfaces.

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