|JKMRS Volume 21, No 1, pp 20; Backbone Assignment of Phosphor...|
|2017년 03월 20일 / 조회수: 260|
Backbone Assignment of Phosphorylated Cytoplasmic Domain B of Mannitol Transporter IIMtl in Thermoanaerobacter Tengcongensis
Ko On Lee and Jeong-Yong Suh*
Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea
Abstract The cytoplasmic domains A and B of the mannitol transporter enzyme IIMtl are covalently linked in Escherichia coli, but separately expressed in Thermoanaerobacter Tengcongensis. The phosphorylation of domain B (TtIIBMtl) substantially increases the binding affinity to the domain A (TtIIAMtl) in T. Tengcongensis. To understand the structural basis of the enhanced domain-domain interaction by protein phosphorylation, we obtained NMR backbone assignments of the phospho-TtIIBMtl using a standard suite of triple resonance experiments. Our results will be useful to monitor chemical shift changes at the active site of phosphorylation and the binding interfaces.