JKMRS Volume 20, No 4, pp 129; Structural Change in Transmembr... | |
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2016년 12월 20일 / 조회수: 594 | |
Structural Change in Transmembrane Region of Syndecan-4 by Mutation
Sung-Sub Choi†, Ji-Sun Kim†, Ji-Ho Jeong and Yongae Kim*
Department of Chemistry, Hankuk University of Foreign Studies, Yong-in, 449-791, Korea Transmembrane(TM) proteins are closely related to
transport, channel formation, signaling, cell to cell interaction, so they are
the crucial target of modern medicinal
drugs. In order to study the structure
and function of these TM proteins, it is important to prepare reasonable
amounts of proteins. However, their preparation is seriously difficult and time-consuming due to
insufficient yields and low solubility of TM proteins.
We tried to produce large amounts of Syndecan-4
containing TM domain(SDC4-TM) that is related to the wound healing and tumor.
Also, mutated SDC4-TM was studied to investigate structural change by
modification of dimerization motif. We performed the structure determination by
the Polarity Index at Slanted Angle (PISA) wheel pattern analysis based on 15N-1H
2D SAMPI-4 solid-state NMR of SDC4-TM and computational modeling using
Discovery Studio 2016. |
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첨부파일 | 5_김용애.pdf |