투고논문

HR-MAS NMR Technique for Metabolic Profiling of Powdery Ginseng

Dahye Yoon¹, Ick-Hyun Jo² and Suhkmann Kim^1,*

¹Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University, 2, Busandaehak-ro 63beong-gil, Geumjeong-gu, Busan, 46241,  Republic of Korea

²Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of Korea

Received Aug 20, 2016; Revised Sep 10, 2016; Accepted Sep 19, 2016

Abstract Ginseng is used as a medicinal ingredient. The quality control of species, age, origin and manufacturing process is important. The metabolome of ginseng about quality was studied in many reports. Almost studies carried out the extract of ginseng, however, the reproducibility cannot be obtained using extracted sample. In this study, powdery ginseng samples were analyzed using high resolution-magic angle spinning nuclear magnetic resonance (HR-MAS NMR)-based metabolomics except extraction step. Sample was measured three times using 600 MHz NMR spectrometer equipped with nano probe. Reproducibility can be enhanced using this method and the metabolic profiles of ginseng were identified and quantified.

Keywords Nuclear magnetic resonance (NMR), Metabolomics, High resolution magic angle spinning (HR-MAS), Ginseng