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JKMRS Volume 20, No 1, pp 22;Expression, Purification and Char...
2016년 03월 19일 / 조회수: 631

Expression, Purification and Characterization of the BLM binding region of human Fanconi Anemia Group J Protein

 

 

Kyuho Yeom and Chin-Ju Park*

 

 

Department of Chemistry, Gwangju Institute of Science and Technology, Gwangju, 61005, South Korea

 

 

 Received Dec 18, 2015; Revised Jan 15, 2016; Accepted Feb 19, 2016

 


Abstract

 

FANCJ is a DNA helicase which contributes genome stability by resolving G-quadruplex DNA from 5’ to 3’ direction. In addition to main ATPase helicase core, FANCJ has the protein binding region at its C-terminal part. BRCA1 and BLM are the binding partner of FANCJ and these protein-protein interactions contribute genomic stability and the proper response to replication stress. As the first attempt for studying FANCJ-BLM interaction, we prepared BLM binding region of FANCJ and characterized with CD and NMR spectroscopy. FANCJ (881-941) with N-ter 6xHis was purified as the oligomer. Secondary structure prediction based on CD data revealed that FANCJ (881-941) composed with β sheet, turn and coils.1H-15N HSQC spectra showed nonhomogeneous peak intensities with less number of peaks comparing than the number of amino acids in the construct. It indicated that optimization should be necessary for detailed further structural studies.

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