JKMRS Volume 19, No 3, pp 143; Proper NMR methods for studying... | |
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2015년 12월 19일 / 조회수: 625 | |
Proper NMR
methods for studying RNA thermometers Won-Je
Kim and Nak-Kyoon Kim* Advanced Analysis Center, Korea Institute of Science
and Technology, 39-1, Hawolgok-Dong Seongbuk-Gu, 136-791, Republic of Korea Received Sep 20, 2015; Revised Nov 04, 2015; Accepted Nov 22, 2015
Abstract In
some pathogenic bacteria, there are RNA thermometers, which regulate the
production of virulence associated factors or heat shock proteins depending on
temperature changes. Like a riboswitches, RNA thermometers are located in the
5’-untranslated region and involved translational gene regulatory mechanism. RNA thermometers block the
ribosome-binding site and start codon area under the 37℃ within their secondary
structure. After bacterial infection, increased the temperature in the host
causes conformations changes of RNA, and the ribosome-binding site is exposed
for translational initiation. Because structural differences between open and
closed forms of RNA thermometers are mainly mediated by base pairing changes, NMR
spectroscopy is a very useful method to study these thermodynamically changing
RNA structure. In this review, we briefly provide a fundamental function of RNA
thermometers, and also suggest a proper NMR experiments for studying RNA
thermometers.
Keywords RNA
thermometer, NMR methods, thermodynamic structure
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첨부파일 | 8김원제.pdf |