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JKMRS Volume 19, No 3, pp 143; Proper NMR methods for studying...
2015년 12월 19일 / 조회수: 625

Proper NMR methods for studying RNA thermometers

 

 

Won-Je Kim and Nak-Kyoon Kim*

 

 

Advanced Analysis Center, Korea Institute of Science and Technology, 39-1, Hawolgok-Dong

Seongbuk-Gu, 136-791, Republic of Korea

 

 

 

 

Received Sep 20, 2015; Revised Nov 04, 2015; Accepted Nov 22, 2015

 

 

 

 

Abstract In some pathogenic bacteria, there are RNA thermometers, which regulate the production of virulence associated factors or heat shock proteins depending on temperature changes. Like a riboswitches, RNA thermometers are located in the 5’-untranslated region and involved translational gene regulatory mechanism. RNA thermometers block the ribosome-binding site and start codon area under the 37 within their secondary structure. After bacterial infection, increased the temperature in the host causes conformations changes of RNA, and the ribosome-binding site is exposed for translational initiation. Because structural differences between open and closed forms of RNA thermometers are mainly mediated by base pairing changes, NMR spectroscopy is a very useful method to study these thermodynamically changing RNA structure. In this review, we briefly provide a fundamental function of RNA thermometers, and also suggest a proper NMR experiments for studying RNA thermometers.

 

Keywords RNA thermometer, NMR methods, thermodynamic structure

 

 

 

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