투고논문
| JKMRS Volume 19, No 1, pp 49-53; Backbone assignments of 1H, 1... | |
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| 2015년 06월 20일 / 조회수: 790 | |
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Backbone assignments of 1H,
15N and 13C resonances and secondary structure prediction
of MRA1997 from Mycobacterium
tuberculosis H37Rv Hyojung Kim, Yena Kim,
Ki-Young Lee and Bong-Jin Lee* Research Institute of Pharmaceutical Sciences,
College of Pharmacy, Seoul National University, San 56-1, Sillim-Dong,
Kwanak-Gu, Seoul 151-742, Korea Received April 15, 2015; Revised May 11, 2015; Accepted
June 1, 2015 Abstract MRA1997 is a
76-residue conserved hypothetical protein of Mycobacterium tuberculosis H37Ra,
one of the most pathogenic bacterial species and the causative agent of
tuberculosis. In this study, the sequence–specific backbone resonance
assignment of MRA1997 was performed using NMR spectroscopy. Approximately 88.3%
of the total resonances could be unambiguously assigned. By analyzing
deviations of the Cα and Cβ chemical shift values, the secondary structure of
MRA1997 was calculated. The result revealed that secondary structure of MRA
1997 consists of one α-helix and five β-sheets. Our structural study will be a
footstone towards the characterization of the three-dimensional structure of
MRA1997. Keywords
MRA1997, a5u406, Mycobacterium tuberculosis, NMR,
secondary structure |
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