JKMRS Volume 19, No 1, pp 42-48; 1H, 15N, and 13C backbone ass... | |
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2015년 06월 20일 / 조회수: 626 | |
1H, 15N, and 13C backbone assignments
and secondary structure of the cytoplasmic domain A of mannitol trasporter IIMannitol
from Thermoanaerobacter Tencongensis phosphotransferase
system Ko-On Lee and
Jeong-Yong Suh* Department of Agricultural Biotechnology, College of
Agriculture and Life Sciences, Seoul National University, San 56-1,
Shillim-Dong, Kwanak-Gu, Seoul 151-742, Korea Received May 4, 2015; Revised May 21, 2015; Accepted June
03, 2015 Abstract The mannitol
transporter Enzyme IIMtl of the bacterial phosphotransferase system
has two cytoplasmic phosphoryl transfer domains IIAMtl and IIBMtl.
The two domains are linked by a flexible peptide linker in mesophilic bacterial
strains, whereas they are expressed as separated domains in thermophilic
strains. Here, we carried out backbone assignment of IIAMtl from
thermophilic Thermoanaerobacter
Tencongensis using a suite of heteronuclear triple resonance NMR
spectroscopy. We have completed 94% of the backbone assignment, and obtained secondary
structural information based on torsion angles derived from the chemical
shifts. IIAMtl of Thermoanaerobacter
Tencongensis is predicted to have six b strands and six a helices, which is
analogous to IIAMtl of Escherichia
coli. Keywords Enzyme IIMtl, mannitol transporter, phosphotransferase
system, Thermoanaerobacter Tencongensis, |
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첨부파일 | 7서정용.pdf |