투고논문


JKMRS Volume 19, No 1, pp 42-48; 1H, 15N, and 13C backbone ass...
2015년 06월 20일 / 조회수: 626

1H, 15N, and 13C backbone assignments and secondary structure of the cytoplasmic domain A of mannitol trasporter IIMannitol from Thermoanaerobacter Tencongensis phosphotransferase system

 

 

Ko-On Lee and Jeong-Yong Suh*

 

 

Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu, Seoul 151-742, Korea

 

 

 

 

Received May 4, 2015; Revised May 21, 2015; Accepted June 03, 2015

 


Abstract   The mannitol transporter Enzyme IIMtl of the bacterial phosphotransferase system has two cytoplasmic phosphoryl transfer domains IIAMtl and IIBMtl. The two domains are linked by a flexible peptide linker in mesophilic bacterial strains, whereas they are expressed as separated domains in thermophilic strains. Here, we carried out backbone assignment of IIAMtl from thermophilic Thermoanaerobacter Tencongensis using a suite of heteronuclear triple resonance NMR spectroscopy. We have completed 94% of the backbone assignment, and obtained secondary structural information based on torsion angles derived from the chemical shifts. IIAMtl of Thermoanaerobacter Tencongensis is predicted to have six b strands and six a helices, which is analogous to IIAMtl of Escherichia coli.

 

Keywords Enzyme IIMtl, mannitol transporter, phosphotransferase system, Thermoanaerobacter Tencongensis,

첨부파일 7서정용.pdf