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JKMRS Volume 17, No 1 40-46; A new approach to quantify paraqu...
2013년 06월 20일 / 조회수: 491

A new approach to quantify paraquat intoxication from postmortem blood sample by using 1H qNMR method

 

 

Ran Seon Hong1, Hwang Eui Cho1, Dong Woo Kim2, Sang Hee Woo2, Sanggil Choe2, Suncheun Kim1,2, Jin Tae Hong1, Dong Cheul Moon1*

 

 

1College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea

 2 National Forensic Service, 305-348, Korea

 

 

Received May 28, 2013; Revised June 10, 2013; Accepted June 12, 2013

 


Abstract   For a case study of suspected paraquat intoxication, we developed a simple and rapid method of 1H qNMR to determine the mili-molar amount of paraquat in postmortem blood samples. There were no interfering signals from endogenous compounds in the chemical shift of paraquat and diquat (internal standard). The amount of sample used ranged from 0.25 mM to 10.0 mM. Diquat, which has similar physicochemical properties with paraquat, was chosen as an internal standard. The NMR experimental conditions, relaxation delay time and CPMG spin-echo pulse sequence were optimized. The developed method was validated in terms of specificity, accuracy, precision, matrix effect, recovery, limit of detection (LOD), and low limit of quantification (LLOQ). The proposed qNMR method provided a simple and rapid assay for the identification and quantification of the quaternary ammonium herbicide, “paraquat” in postmortem blood samples. This method was tested by using the blood from the heart of a man who was intoxicated with paraquat. In this particular case, the level of paraquat was 1.07 mM in the blood. For the determination of quaternary ammonium herbicides, qNMR could also be used to provide a better understanding of the currently available techniques.

 

Keywords   Paraquat, 1H qNMR, whole blood

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