투고논문

Abstract The M9 minimal medium is widely used for producing isotope-labeled proteins in Escherichia coli (E. coli). However, the original M9 formulation does not fully support optimal E. coli growth. To address this limitation, several modified versions featuring increased potassium concentrations, enhanced buffering capacity, and other improvements have been developed to promote robust growth and high-yield protein production. In this protocol, we aimed to identify the best conditions that support E. coli survival and adaptation by comparing the performance of modified minimal medium variants with the original M9 medium, and proposed the optimized formulation of a minimal medium (M-opt) suitable for efficient isotope-labeled protein production.

Keywords Buffering capacity, Escherichia coli, minimal media, potassium, trace metal

* Address correspondence to: Kyoung-Seok Ryu, Biopharmaceutical Research Center, Korea Basic Science Institute, Cheongju 28119, Repulic of Korea, Tel: 82-43-240-5064; Fax: 82-43-240-5059; E-mail: ksryu@kbsi.re.kr