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JKRMS Volume 27, No 3, pp 17, Backbone assignment of the intri...
2023년 09월 20일 / 조회수: 123

Backbone assignment of the intrinsically disordered N-terminal region of Bloom syndrome protein

 

 

Min June Yang and Chin-Ju Park*

 

 

Department of Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea

 

 

 

Received Sep 4, 2023; Revised Sep 15, 2023; Accepted Sep 18, 2023

 

Abstract Bloom syndrome protein (BLM) is a pivotal RecQ helicase necessary for genetic stability through DNA repair processes. Our investigation focuses on the N-terminal region of BLM, which has been considered as an intrinsically disordered region (IDR). This IDR plays a critical role in DNA metabolism by interacting with other proteins. In this study, we performed triple resonance experiments of BLM220-300 and presented the backbone chemical shifts. The secondary structure prediction based on chemical shifts of the backbone atoms shows the region is disordered. Our data could help further interaction studies between BLM220-300 and its binding partners using NMR.

 

Keywords Bloom syndrome protein, backbone assignment, intrinsically disordered region

 

* Address correspondence to: Chin-Ju Park, Department of Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea, Tel: 82-62-715-3630; Fax: 82-62-715-2866; E-mail: cjpark@gist.ac.kr

첨부파일 1_ParkCJ.pdf