투고논문
| JKMRS Volume 25, No 3, pp 33, Metabolite analysis in the type ... | |
|---|---|
| 2021년 09월 20일 / 조회수: 407 | |
|
Metabolite analysis in
the type 1 diabetic mouse model
Sung Jean Park*
College of Pharmacy and Gachon Institute of
Pharmaceutical Sciences, Gachon University, 191 Hambakmo-ero, Yeonsu-gu,
Incheon 21936, Republic of Korea
Received September 2, 2021; Revised
September 14, 2021; Accepted September 14, 2021
Abstract Type 1 diabetes mellitus (T1DM) is caused by insufficient
production of insulin, which is involved in carbohydrate metabolism. Type 2
diabetes mellitus (T2DM) has insulin resistance in which cells do not respond
adequately to insulin. The purpose of this study was to estimate the characteristics
of type 1 diabetes using streptozotocin-treated mice (STZ-mouse). The sera
samples were collected from the models of hyperglycemic mouse and healthy mouse.
Based on the pair-wise comparison, five metabolites were found to be
noticeable: glucose, malonic acid, 3-hyroxybutyrate, methanol, and tryptophan.
It was very natural glucose was upregulated in STZ-mouse. 3-hyroxybutyrate was
also increased in the model. However, malonic acid, tryptophan, and methanol
was downregulated in STZ-mouse. Several metabolites acetoacetate, acetone, alanine,
arginine, asparagine, histidine, lysine, malate, methionine, ornithine, proline,
propylene glycol, threonine, tyrosine, and urea tended to be varied in
STZ-mouse while the statistical significance was not stratified for the
variation. The multivariate model of PCA clearly showed the group separation
between healthy control and STZ-mouse. The most significant metabolites that
contributed the group separation included glucose, citrate, ascorbate, and
lactate. Lactate did not show the statistical significance of change in t-test
while it tends to down-regulated both in DNP and Diabetes.
Keywords Diabetes, Type I, Metabolite, NMR, Streptozotocin
|
|
| 첨부파일 | 1_ParkSJ.pdf |