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JKMRS Volume 25, No 1, pp 1, Theoretical and practical feature...
2021년 03월 20일 / 조회수: 118

 

 

Theoretical and practical features of J-scaled distortion-free HSQC

 

 

Jin Wook Cha1,* and Sunghyouk Park2

 

 

1Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products, Gangneung 25451, Republic of Korea

2Natural Product Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea

 

 

 

 

Received Mar 19, 2021; Revised Mar 19, 2021; Accepted Mar 19, 2021


Abstract Employing of 13C stable-isotopes in NMR metabolomics can give unique splitting patterns and coupling constants information originated from 13C-13C coupling interaction that provide an important structural information regarding the cellular metabolic process. But it has been known that an undesirable signal distortion in 2D heteronuclear correlation study, due to 13C-13C interaction, hampers an analysis of the coupling information. Recently, we proposed J-scaled distortion-free heteronuclear single-quantum coherence (HSQC) sequence which provides a distortion-free 13C-13C coupling information with a selective resolution enhancement of JCC splitting. In this paper, we dicuss theoretical aspect and practical feature of J-scaled HSQC pulse sequence. The conceptual explanation of orgin of the signal distortion by 13C-13C coupling interaction and design of J-scaled HSQC through exemplified results are provided in brief.

 

 

Keywords 13C-13C coupling, 13C-isotope, HSQC, J-scaling, NMR spectroscopy

 

 

* Address correspondence to: Jin Wook Cha, Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products, Gangneung 25451, Republic of Korea, Tel: 82-33-650-3511; E-mail: elmarit@kist.re.kr


 

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