투고논문

Theoretical and practical features of J-scaled distortion-free HSQC

Jin Wook Cha^1,* and Sunghyouk Park²

¹Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products, Gangneung 25451, Republic of Korea

²Natural Product Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea

Received Mar 19, 2021; Revised Mar 19, 2021; Accepted Mar 19, 2021

Abstract Employing of ¹³C stable-isotopes in NMR metabolomics can give unique splitting patterns and coupling constants information originated from ¹³C-¹³C coupling interaction that provide an important structural information regarding the cellular metabolic process. But it has been known that an undesirable signal distortion in 2D heteronuclear correlation study, due to ¹³C-¹³C interaction, hampers an analysis of the coupling information. Recently, we proposed J-scaled distortion-free heteronuclear single-quantum coherence (HSQC) sequence which provides a distortion-free ¹³C-¹³C coupling information with a selective resolution enhancement of J_CC splitting. In this paper, we dicuss theoretical aspect and practical feature of J-scaled HSQC pulse sequence. The conceptual explanation of orgin of the signal distortion by ¹³C-¹³C coupling interaction and design of J-scaled HSQC through exemplified results are provided in brief.

Keywords ¹³C-¹³C coupling, ¹³C-isotope, HSQC, J-scaling, NMR spectroscopy

* Address correspondence to: Jin Wook Cha, Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products, Gangneung 25451, Republic of Korea, Tel: 82-33-650-3511; E-mail: elmarit@kist.re.kr