JKMRS Volume 24, No 4, pp 104, NMR-based Metabolomic Responses... | |
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2020년 12월 20일 / 조회수: 480 | |
NMR-based Metabolomic
Responses of Zebrafish (Danio Rerio) by Fipronil Exposure
Sujin
Lee, Sangah Oh, Seonghye Kim, Wonho Lee, Juyoung Choi, Hani Lee, Yujin Lee,
Department of Chemistry and Chemistry Institute for
Functional Materials,
Received October 15, 2020; Revised
December 11, 2020; Accepted December 11, 2020
Abstract Fipronil, the phenylpyrazole insecticide, is effective
and used in various fields. Especially, fipronil was reliable because it was
known to be specific on invertebrate animals than vertebrate animals including
mammals. However, fipronil had potential risks that affect vertebrate animals
as it blocks the gamma-aminobutyric acid (GABA) receptors that also exists in
vertebrates as well as invertebrates. Therefore, it was necessary that harmful
effects of fipronil on vertebrates are clarified. For this purpose, the zebrafish
(Danio rerio) were used on behalf of vertebrate animals in present study. The
zebrafish were exposed to 5 µg/L, 25 µg/L, and 50 µg/L of fipronil during 12,
24 and 72 hours. To closely observe toxic process, 12 hours and 24 hours of
additional time point were set in the exposure test. Nuclear
magnetic resonance (NMR)-based metabolomics is an approach to detect metabolic
changes in organism resulted from external stimuli. In this study, NMR-based
metabolomics showed the metabolic changes in zebrafish caused by fipronil
exposure. Metabolic analysis revealed that fipronil interfered
with energy metabolism and decreased the antioxidant ability in zebrafish.
Antioxidant ability decline was remarkable at high exposure concentration. In
addition, metabolic analysis results over time suggested that reactions for
alleviating the excessive nerve excitation occurred in zebrafish after fipronil
exposure. Through this study, it was elucidated that the adverse effects of
fipronil on vertebrate animals are evident. The risk of fipronil on vertebrates
can be no longer ignored. Moreover, this study has a meaning of practically
necessary research for organism by examining the effects of fipronil at low
concentrations existed in real environment.
Keywords High resolution-magic angle spinning, nuclear magnetic resonance spectroscopy, metabolomics, Danio rerio, Fipronil
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첨부파일 | 02-JKMRS_KimSM.pdf |