투고논문

Solvent-localized in-situ NMR Monitoring
by Intermolecular Single-quantum Coherence Study

Jin Wook Cha^1,* and Sunghyouk Park²

¹Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products, Gangneung 25451, Republic of Korea

²Natural Product Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea

Received December 11, 2020; Revised December 18, 2020; Accepted December 18, 2020

Abstract A new NMR method to monitor solvent-localized NMR signals in the two-phase liquid system is suggested. This method based on intermolecular single-quantum coherence (iSQC). Here, we exploited the feature of the local action of distant dipolar field (DDF) effect in order to filter out specific NMR signals dissolved in different solvents. This solvent specific iSQC spectroscopy was carried out on a model two-phase liquid system (D-glucose in water/palmitic acid in chloroform), and showed solvent-localized NMR signals. We believe our approaches might be useful in metabolic analysis such as two-phase liquid extraction scheme for labile chemical species.

Keywords DDF, iSQC, mixture analysis, two-phase liquid extraction

* Address correspondence to: Jin Wook Cha, Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products, Gangneung 25451, Republic of Korea, Tel: 82-33-650-3511; E-mail: elmarit@kist.re.kr